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Tevion crm 2005 user manual: In the following, tevion crm 2005 user manual is a bit out. But if you are looking for version with. in-lab thymic organoid culture assay \[[@B33-viruses-12-00464]\]. In addition to the thymic organoid assay, this approach is also valid for measuring viral latency in reconstituted human lymphoid tissues, such as tonsils, lymph nodes, mucosal-associated lymphoid tissue, and intestine \[[@B33-viruses-12-00464],[@B34-viruses-12-00464]\].

5. Future Perspectives {#sec5-viruses-12-00464}
======================

Immune monitoring assays have become important tools for the diagnosis, prognosis, and monitoring of disease treatments. Evaluating the levels of broadly neutralizing antibodies is an important part of the development of an effective vaccine. Some serum neutralizing antibody levels have been correlated with protection \[[@B35-viruses-12-00464]\], and neutralizing antibody levels have been correlated with protection in other mucosal tissues \[[@B36-viruses-12-00464],[@B37-viruses-12-00464],[@B38-viruses-12-00464]\]. However, the neutralizing antibody levels in humans has not been consistently correlated with protection in many clinical trials \[[@B10-viruses-12-00464],[@B39-viruses-12-00464],[@B40-viruses-12-00464],[@B41-viruses-12-00464],[@B42-viruses-12-00464],[@B43-viruses-12-00464]\]. In the vast majority of trials, sera were tested by virus neutralization assays using few human isolates. Due to the high degree of antigenic variation of HIV-1, human isolates are not a good representation of the circulating variants. The failure to correlate antibody responses in humans with protection has been attributed to the genetic heterogeneity among the viruses in circulation \[[@B10-
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