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Formalin-fixed, paraffin-embedded tissue sections were deparaffinized with xylene and rehydrated in ethanol. Slides were pretreated with H~2~O~2~ and antigen retrieval for half an hour at 97°C, then blocked with 1.5% normal donkey serum for 30 mins. Slides were then incubated with anti-CD133/2-APC antibody (1:100, Novus Biologicals, 1:200, Abcam, Cambridge, MA), anti-CD133/2-APC antibody (1:50, Santa Cruz), anti-tyrosine hydroxylase (TH) antibody (1:50, Abcam) or anti-NeuN antibody (1:200, Millipore, Billerica, MA) overnight at 4°C. After staining, sections were washed with PBS for three times, followed by incubation with diluted biotinylated secondary antibody (1:200, Jackson Immunoresearch, West Grove, PA), and then further stained with HRP-avidin (1:100, Sigma, St. Louis, MO) and DAB (Thermo Fisher Scientific, Waltham, MA), and counterstained with haematoxylin. Images were captured with a microscope (Olympus, Tokyo, Japan). All staining intensities were scored by at least two independent researchers in a blinded manner. Immunofluorescence (IF) staining of retinal sections was performed according to the methods described previously \[[@R43]\]. Briefly, frozen sections were prepared and fixed with methanol at room temperature for 3 min, then washed with PBS and permeabil

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How does the ‘2’ command work in using bash in interactive mode?

I can see that using bash as an interactive shell (eg: bash) gives two options when I enter commands.
$ bash
Last login: Thu Mar 14 07:53:26 2018 from 192.168.0.118
$ ls
zsh ~ ~/.bash_profile ~/.bash_logout ~/.inputrc ~/.profile ~
/kern/bin/ls bash-4.2$ ls ~ ~/.bash_profile ~/.bash_logout ~/.inputrc ~/.profile ~

But I can’t seem to understand how this works in the second case.

How does bash know to return two lines of output when I input an ls command?
I thought the input command only returns the first line?

A:

The lines you see when typing a command are only displayed if the command has an expansion. bash uses this to show something like “command-expansion-here”. ls is expanded to a list of matching files to ls -l. Because the command list is printed on 2 lines, the command gets printed twice.

A:

The command has an expansion. It expands to 2 lines of output which is printed to the console.

EU’s decision on sanctions against Belarusian PM Alyaksandr Lukashenka is a sad day for Belarus

On Friday, December 2, the European Union (EU) suspended certain sanctions against Belarus. The decision is prompted by the country’s violation of human rights and the democratic process.

The situation in Belarus can be considered quite optimistic.

The EU has taken a clear stance against the authoritarian Lukashenka regime.

However, the decision on the suspension of the sanctions is only aimed at loosening the means of influence on the Belarusian state. Such an approach shows once again a certain weakness of the EU, as the decision is part of the latest developments on the EU-Ukraine agenda.

On Friday, the EU’s High Representative for Foreign Affairs and Security Policy, Federica Mogherini, confirmed to the press that the decision