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Solution Theory Of Machines R S Khurmi

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A rat model of Duchenne muscular dystrophy has been used to compare patterns of connexin 43 (Cx43) as gap junction proteins of myofibers. First, myofibers were isolated by two-dimensional electrophoresis and screened by use of an antibody directed against the carboxyl terminus of Cx43. Specificity of the antibody was determined by immunoblot analysis of homogenates of nonmuscle cells, neuromuscular junctions (NMJs), and muscle fibers by use of various anti-Cx antibodies. The specificities of the anti-Cx antibodies were determined and proved in the same way. Among the antigens detected in myofibers, two antigens designated alpha and beta are shown to be Cx43 by comparison with molecular mass and isoelectric point. The presence of Cx43 was then investigated in the regenerating muscle after sciatic nerve transection. Total homogen

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