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To analyse the additive effect of Notch and apoptosis inhibition, we used apoptosis inhibitors, respectively. As shown in Fig. [6c,d](#Fig6){ref-type=”fig”}, a combination treatment of 10 μM MG-132 and a 10 μM DAPT for 72 h induced a synergistic increase of cleaved caspase-3 and the frequency of apoptotic cells in Saos-2 cells.

Further, we assessed apoptosis in cells under the condition of combined inhibition of Notch and MG-132 treatment. Our results showed that combined inhibition of Notch and MG-132 treatment resulted in an additive effect in the cell-cycle analysis (Fig. [6b](#Fig6){ref-type=”fig”}). Moreover, additive increase of apoptotic cells after the combined inhibition of Notch and MG-132 treatment were confirmed (Fig. [6e](#Fig6){ref-type=”fig”}). This result clearly demonstrated that inhibition of Notch pathway in MG-132-treated
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